Cell Biology Antibodies You Can Rely On!

Reliable antibodies for reproducible data

The ability to generate reproducible data with the use of antibodies is under scrutiny in the scientific community. Therefore, PROGEN started a quality initiative to serve specifically to today´s urgent customer need for high quality and reliability for the life science research community.

  • Established manufacturer since 1983
  • DIN ISO 13485 certified company since 2010
  • Antibodies from scientific collaborations with leading research groups
  • >800 antibodies for your cell biology discoveries

Thorough antibody validation

Application confirmed

  • Evaluation of antibody performance in western blot using specific WB positive controls
Highly published

  • PROGEN antibodies are among the most published in biomedical and cell biology literature
External validation

  • External quality control of IHC, ICC and IF antibodies
  • Validation of every new antibody lot on relevant tissue sections or cells
Validated by epitope mapping

  • Generation of dependable data to characterize antibody selectivity and specificity
  • Testing for cross-reactivity
  • Approach has been "highly commended" in the 2018 CiteAb validation award

Validation by epitope mapping

 
In order to provide optimal data on selectivity, specificity and cross-reactivity of our western blot antibodies, PROGEN utilizes the peptide mapping technology of our collaborator PEPperPRINT GmbH to validate selectivity and specificity of the majority of our western blot antibodies.


Method

  • generation of a peptide microarray with peptides of 15 aa of the relevant target antigen (each with 14 aa overlap)
  • incubation with target antibody and fluorescently labelled secondary antibody
  • spot pattern of immunoreaction represents binding of the antibody to specific peptide fragments    
  • identification of a stretch of adjacent peptides with consensus motif representing the binding epitope by microarray scan and intensity plot

This unique peptide mapping technique allows us to precisely identify the binding epitope sequence of antibodies along with a broad specificity profile. By using this thorough validation method for the majority of PROGENs western blot antibodies we want to underline the high quality of our antibodies and offer additional value to the research community.

For more information, check our poster"Buying an antibody is like playing the lottery - Validation of keratin antibodies by epitope mapping"

 

Western blot positive controls

Take control of your western blot! PROGEN offers a range of western blot positive controls for a selection of its antibodies. Either available separately or in sets of antibody + validated western blot control.

  • Individual western blot positive controls for selected antibodies
  • Tested and validated controls from whole cell lysate
  • Defined concentration for detection

Browseour western blot positive controls.

    

Interview: How to choose an antibody that works

PROGENs Head of Research & Development gives you an insight on how PROGEN validates its antibodies and what to consider to make the right antibody choice for your research application. Read the full article on How to choose an antibody that works" and get some valuable tips how to avoid countless repeats of testing and optimization.

 

Tutorial: 3 steps to choose the right antibody

Only antibodies that fit your application will lead to successful experiments.
The following infographicsummarizes three major aspects that will guide you in selecting the right antibody for your research.

 

Information on individual antibodies

Keratins - more than structural components

Keratins are a large family of structural proteins expressed in a highly tissue- and differentiation-specific manner. They are involved in establishing apico-basal polarization, regulating motility and cell size and even play a role in complex cellular events such as protein synthesis, membrane traffic or cell signaling. Highly specific keratin antibodies are useful tools as ubiquitous epithelial markers to study the multiple roles of keratin in cell biology and cancer diagnosis & prognosis.

Learn more about our keratin antibodies

p53 mouse monoclonal antibodies

The p53 protein is a cell cycle regulator and as such a key tumor suppressor that induces growth arrest or apoptosis in tumor cells of different origin. The molecule is under intense study in oncological and cell biology research and reliable antibodies are required for immunohistology and western blot.

Learn more about our p53 antibodies

AAV antibodies

Adeno-Associated Virus (AAV) is a nonpathogenic virus that is widely used as vector in gene therapy. In view of this scientific and preclinical significance, PROGEN is providing AAV antibodies that specifically recognize intact virus capsids as well as antibodies specific to AA-viral subunits and replicases.

Learn more about our AAV antibodies

 

Perilipin antibodies

PERILIPINs are proteins of the membrane surrounding lipid droplets (LD). Perilipin antibodies are useful for the characterization of LD subpopulations with their multifunctional properties in health and disease, including lipid transport, lipogenesis and lypolysis.

Learn more about our perilipin antibodies

p62/SQSTM1 antibodies

The p62 protein plays a central role in ubiquitin-associated degradation and autophagy where it acts as a selective autophagy receptor, assembled in filamentous polymers and shuttling ubiquitinylated proteins to the autophagosomes.

Learn more about PROGEN's p62 antibodies

 

Use PROGEN's antibody selector to find what you need!