protag - Single Domain Antibodies

Single domain antibodies (sdAb) consist of a single, monomeric variable antibody domain, representing the antigen binding domain of a common antibody, composed of two heavy and two light chains. Since the variable domain is responsible for the specific antigen recognition, sdAbs show the same selectivity and specificity as common antibodies. The small size of sdAbs leads to an increased stability at high temperatures and decreased sensitivity to detergents and high concentrations of urea. The sdAb peptide chain of about 110aa usually originates from the variable chain of either a common IgG or a heavy chain antibody. The protag single domain antibodies are derived from llamas or alpacas and are recombinantly produced in E.coli.

     

Features

  • 10x smaller than conventional antibody
  • Significantly lighter than conventional antibody
  • High affinity to target protein
  • Minimal linkage error
  • Single molecule localization
  • Labelled with defined number of fluorophore molecules
  • Recombinant production in E.coli

Benefits

  • Improved tissue permeability & access to target proteins
  • close proximity, nanometer spacial resolution & less distance related misleading signals
  • Improved signal/noise ratio
  • Suitable for super resolution microscopy
  • Superior identification of small amounts of antigens
  • highly reproducible & less batch-to-batch variability

protag - For Better Research

PROGEN offers a selection of different protag antibodies for a broad range of applications
(protag-HiSec, protag-HiRes and protag-HiPur).   

protag-HiRes

  • fluorescently labeled primary antibodies 
  • against four fluorescent tags and their variants (GFP, RFP, mScarlet-i and TagFP)
  • available with one (protag-HiRes X1) or two (protag-HiRes X2) fluorophores linked to a single protag molecule 
  • available as mix of two different protag molecules targeting the same protein, both labeled with two fluorophores            --> up to four fluorophore molecules per target protein (protag-HiRes X4)
  • available with 10 different fluorophores (Abberior® Star 488, Abberior® Star RED, Abberior® Star 580,                          Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Cy3 and Cy5) 

Learn more about our protag-HiRes antibodies

protag-HiSec

  • fluorescently labeled secondary sdAb
  • against mouse, rabbit, guinea pig IgG and chicken IgY, respectively
  • two fluorophore molecules linked to one protag molecule (protag-HiSec X2
  • available with 10 different fluorophores (Abberior® Star 488, Abberior® Star RED, Abberior® Star 580,                          Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N,Cy3 and Cy5) 

Learn more about our protag-HiSec antibodies

protag-HiPur

  • immobilized on 4% cross-linked agarose beads
  • against five of the most common tags used for tag-fusion proteins (GST, MBP, GFP, RFP and TagFP)
  • suitable for immunoprecipitation (IP) and mass spectrometry (MS)
  • no leakage of heavy and light chain during elution with SDS buffer
  • compatible with highly stringent buffers
  • high binding capacity because of small size of the sdAb and high accessibility

Learn more about our protag-HiPur agarose beads